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Example 2, Flow Cytometry

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Example of a CoA Report Chart for Characterization of a peripheral blood leukemic sample

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Customer:………………………………………………………………………………………..   Donor Sample#: Deidentified ………………………………………

Address Line 1:………………………………………………………………………………….             Age:……years

Address Line 2:…………………………………………………………………………………     SEX:…………………….                                                                                                 

Lab Director:…………………………………………………………………………………….              Sample Collection Date: …..-…..-…………  

Leukemia/Lymphoma Phenotyping Evaluation by Flow Cytometry

Leukemia/Lymphoma Phenotype & Impression               

Sample type: Peripheral blood

Impression: Atypical myeloid blasts identified accounting for 92% of the viable leukocytes, favor

Acute Myeloid leukemia.

Differential:

Lymphocytes (4%)

Monocytes (1%)

Granulocytes (3%)

Atypical cells (92%)

Atypical cells: Myeloid blasts

Immunophenotype:

*Positive: partial CD4, CD7, CD13, CD33, partial CD34, variable CD38, dim CD45, CD117, CD123, HLA-DR, MPO

*Negative: CD2, CD5, CD10, CD11b, CD14 (Mo2), CD15, CD19, CD20, CD41, CD42a, CD42b, CD61, CD64, CD71, CD56, TDT, Cyt CD3

* Phenotypic abnormalities: CD7 positive

Lymphocytes:

B-cell (27%)

T-cell (57%)

NK-cell (11%)

Kappa / Lambda ratio: 1.5, Polytypic

CD4 /CD8 ratio:  normal at 1.7

Nk-cells: no abnormality

Monocytes: no abnormality, left shifted with partial CD14

Myeloid cells: no abnormality, left shifted with partial CD10, partial CD11b, partial CD16

Vitality; (95%)

Disclaimers: None

Markers run: CD1, CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD11b, CD13, CD14 (Mo2), CD14 (MY4), CD16, CD19, CD20, CD23, CD33, CD34, CD38, CD45, CD56, CD64, CD117, HLA-DR, Kappa, Lambda, MPO, TdT, CD200, CD57, CD123, Cyt CD3

These results have been reviewed and approved by (name, function):……………………………………………………………………………………………………

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