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Example 2, Flow Cytometry
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Example of a CoA Report Chart for Characterization of a peripheral blood leukemic sample
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Customer:……………………………………………………………………………………….. Donor Sample#: Deidentified ………………………………………
Address Line 1:…………………………………………………………………………………. Age:……years
Address Line 2:………………………………………………………………………………… SEX:…………………….
Lab Director:……………………………………………………………………………………. Sample Collection Date: …..-…..-…………
Leukemia/Lymphoma Phenotyping Evaluation by Flow Cytometry
Leukemia/Lymphoma Phenotype & Impression
Sample type: Peripheral blood
Impression: Atypical myeloid blasts identified accounting for 92% of the viable leukocytes, favor
Acute Myeloid leukemia.
Differential:
Lymphocytes (4%)
Monocytes (1%)
Granulocytes (3%)
Atypical cells (92%)
Atypical cells: Myeloid blasts
Immunophenotype:
*Positive: partial CD4, CD7, CD13, CD33, partial CD34, variable CD38, dim CD45, CD117, CD123, HLA-DR, MPO
*Negative: CD2, CD5, CD10, CD11b, CD14 (Mo2), CD15, CD19, CD20, CD41, CD42a, CD42b, CD61, CD64, CD71, CD56, TDT, Cyt CD3
* Phenotypic abnormalities: CD7 positive
Lymphocytes:
B-cell (27%)
T-cell (57%)
NK-cell (11%)
Kappa / Lambda ratio: 1.5, Polytypic
CD4 /CD8 ratio: normal at 1.7
Nk-cells: no abnormality
Monocytes: no abnormality, left shifted with partial CD14
Myeloid cells: no abnormality, left shifted with partial CD10, partial CD11b, partial CD16
Vitality; (95%)
Disclaimers: None
Markers run: CD1, CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD11b, CD13, CD14 (Mo2), CD14 (MY4), CD16, CD19, CD20, CD23, CD33, CD34, CD38, CD45, CD56, CD64, CD117, HLA-DR, Kappa, Lambda, MPO, TdT, CD200, CD57, CD123, Cyt CD3
These results have been reviewed and approved by (name, function):……………………………………………………………………………………………………
Signature: